ISBN: 1444336967 9781444336962

Category: Science / Life Sciences / Zoology / Entomology

Page count: 368

<p>This helpful guide is self-contained, holding all of the information you will need for the preparation of histological slides of all types of insect tissues. It explains the essential procedures for their preparation, providing step-by-step information on each phase, for example, on fixation, dehydrating, clearing, embedding, and staining. <p>The laboratory manual explains the cutting-edge methodology carried out today, and provides a thorough collection of both historically established and modern insect histology techniques side-by-side. It is the first book to present these various techniques appropriate to insects. <p>Key issues covered include: <ul><li>the use of genetic markers in insect histology;</li><li>important approaches for the preparation of tissues and organs for SEM and TEM Fluorescence;</li><li>new immunohistochemical techniques;</li><li>histological problems encountered in insect tissues such as sclerotized chitin, yolk-laden eggs, chromosomes, genitalia, etc., and how to deal with them.</li></ul> <p>Photographs show the likely results of procedures, components of the process, and advantages of the use of particular approaches or compounds. As a comprehensive resource, this book is for students and researchers in entomology, systematics, developmental biology, insect cell biology, and morphology, wanting a clear introduction to the procedures for the histological display of insect tissues.