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Analyzing Trogocytosis of T Lymphocytes by Flow Cytometry and Confocal Microscopy

by Alicia Zink, Simon Zenke, Teresa Wiese, Niklas Beyersdorf, Tim Lämmermann, Jan Rohr · 2023

ISBN:  Unavailable

Category: Unavailable

Page count: Unavailable

Abstract: Here, we present a protocol to examine the mechanisms underlying the intercellular transfer of transmembrane molecules, termed trogocytosis, and the fate of transferred molecules. We describe the steps needed from T lymphocyte isolation, via co-culture with cells expressing the ligand of interest, to cell harvest and subsequent staining for flow cytometry and confocal microscopy. Furthermore, we showcase critical parameters and pitfalls, which allow easy adaptation of the protocol to investigate trogocytosis of various cell surface receptors in different cell types