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Protonema Induction, Transient Transformation, and Protoplast Regeneration in the Peat Moss Sphagnum Papillosum

by Volker Lüth, Alena Kaltenbrunner, Arnaud Pascal, Eva Decker, Ralf Reski · 2023

ISBN:  Unavailable

Category: Unavailable

Page count: Unavailable

Abstract: Sphagnum mosses are important carbon sequesters and emerging model organisms. However, induction and long-term cultivation of thalloid protonema in several species was not achievable so far. Here, we provide protocols for a set of new tools relevant for Sphagnum molecular biology: a new way for Sphagnum protoplast isolation and regeneration, and a first protocol for transient protoplast transformation. Together, these protocols will support the emerging Sphagnum research community in basic and applied science