Antibodies are a critical part of modern medicine, comprising 20% of the novel drugs approved by the FDA in 2015. While there are several platforms available to develop antibodies against a given antigen, they generally take months to carry out or require expensive equipment and extensive technical expertise. Recently, Robinson et al. demonstrated that SHuffle T7-Express cells are able to form disulfide bonds within the cytoplasm, enabling the cytoplasmic production of full-length antibodies. Using this technology, this work develops a novel antibody selection platform using chloramphenicol acetyltransferase (CAT) export to the periplasm via the Tat pathway. Within each cell two constructs are expressed within the cytoplasm, an antibody and the CAT-antigen fusion, which is rapidly exported into the periplasm resulting in chloramphenicol sensitivity. In cases where the expressed antibody displays sufficient binding activity with the antigen, the resulting complex will hinder the export of the CAT-antigen fusion, restoring chloramphenicol resistance. In this study we demonstrate that Tat enabled CAT export reliably reports the binding interaction between various antigens and their antibody cognates. We have further validated this approach by utilizing it to identify known antibody-antigen pairs from a mock library with 100-fold excess of non-specific pairs. Finally, one of the successful antibodyantigen pairs was used to construct a CDR-H3 library for screening with this platform and initial results appear promising. We believe that our platform will provide a simple and cost effective method for rapid discovery of clinically relevant antibodies for use with a wide range of diseases and illnesses.

"The purpose of this study was to describe various kinematic variables of the skating stride. A data set for five collegiate hockey players was completed (mean +/- SD: age = 22.0 +/- 1.0 years, height = 1.77 +/- 0.06 m, weight = 80.0 +/- 8.5 kg). Three velocities were examined on the skating treadmill: slow (12 km/hr), medium (18 km/hr) and fast (24 km/hr). Electrogoniometers at the hip (H), knee (K) and ankle (ANK) were used to acquire angular displacement and velocity profiles. A trend for increasing range of motion and a significant (p

From the website: Rejection. Every writer faces it. But more interesting than the ways writers have been rejected are the ways writers reject. For Paul Beatty, the rejection is of our nation's shameful legacy of racism. In "Looking for Suzanne," Chris Kraus's rejected narrator tries to put the pieces of his enigmatic ex together, while in Claire Vaye Watkins's "The Call," futuristic California seems to have rejected everyone. Translators Richard Pevear and Larissa Volokhonsky refuse to accept that the classic translations of Russian classics are sacred, and have made a career of breathing new life into Anna Karenina, War and Peace, and Selected Stories of Anton Chekhov, among many others. And channeling their spirit, perhaps, we embrace the opportunity to publish one of Chekhov's previously untranslated stories, "Artists' Wives." Not to be outdone, even from the grave, Hemingway weighs in with a pugilistic letter, also previously unpublished. We all know what being rejected feels like. So it seemed like a gift to offer a handful of writers, including Mitchell S. Jackson and Leslie Jamison, the opportunity to pen their own rejection letters. James Patterson, one of the best-selling authors of all time, addresses us all, urging us to reject rejection and rally around the flag of reading. And poet Mary Ruefle has the last word, flat out rejecting Tin House. Ouch. But you, dear readers, must know we'll never reject you.